Isolation and Characterization of Antibacterial Peptides from the Mud-crab, Scyllaserrata

M.I. Hoq; M.U. Seraj; S. Chowdhury

Isolation and Characterization of Antibacterial Peptides from the Mud-crab, Scyllaserrata

2003

M.I. Hoq | M.U. Seraj | S. Chowdhury

Both induced and non-induced hemolymphs were collected from the mud-crab, Scyllaserrata and were subjected to antibacterial assay to investigate the presence ofinducible and constitutive antibacterial protein(s). Induction was done by injectingEscherichia coli ATCC 25922. The induced hemolymph showed antibacterial activityagainst a range of different strains of marine or non-marine gram-positive and gram-negative bacteria including few antibiotic resistant strains, whereas the non-inducedhemolymph was not appeared to be active against those tested. The inducedhemolymph was subjected to SDS-PAGE and seven proteins were detected. Themolecular weight of which were in the range from 36 to 84.5 kDa.<span style="color: #800080"> </span>The hemolymphproteins were fractionated by gel filtration chromatography. Repeated antibacterialassay of the chromatographic fractions against previously proved hemolymph sensitivebacteria showed that only a few fractions were responsible for the activity and that thefraction purified proteins showed better activity than the crude hemolymph. Total fivedifferent proteins were present in all the fractions detected by SDS-PAGE analysis. Themolecular weights were 64 kDa, 61 kDa, 56.5 kDa, 49 kDa and 36 kDa.

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