Bioactivities of Protein Isolated from Marine Sponge, Sigmadocia fibulatus

P. Soundarapandian

Bioactivities of Protein Isolated from Marine Sponge, Sigmadocia fibulatus

2009

P. Soundarapandian

The marine sponge Sigmadocia fibulatus, collected from Tuticorin coast, was studied forantibacterial and antifungal activity of proteins. Sponge species identified based on spicules morphology.Chloroform and aqueous extracts yielded a total amount of 6.8g and 5.5g from 500g of sponge respectively.Crude protein from the sponge was extracted the concentration of 2.82 g/mL in aqueous and 1.93 mg/mL inchloroform extract. The antimicrobial activity of crude extract against bacterial and fungal pathogens showedthe clear inhibition zone against Vibrio cholerae and Aspergillus niger in chloroform extract and aqueousextracts showed clear inhibition zone for Pseudomonas sp. and Candida albicans. Both the extracts exhibitedhemolytic activity which was estimated as 11.09ht/mg for chloroform extract and 9.8ht/mg for aqueous extract.The partial purification of protein is done by using DEAE cellulose. On SDS-PAGE the crude protein yieldedthree well defined bands at 109.9, 28.2, 12.4 KDa in both the extracts. The Fatty acid profile showed thedominance of myristic acid (14.67%) in the case of aqueous and chloroform extracts.

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