Bio-chemical response by elevated salinity stress in two barley lines

Marjan, F. . Dept. of Chemistry; Ashraf, M.Y.; Khalil-ur-Rehman . Dept. of Chemistry

Bio-chemical response by elevated salinity stress in two barley lines

2012

Marjan, F. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry) | Ashraf, M.Y. (Nuclear Inst. for Agriculture and Biology, Faisalabad (Pakistan)) | Khalil-ur-Rehman (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry)

A Lysimeter scale experiment was conducted to determine the effect of elevated salinity on growth parameters and changes in biochemical attributes in 15 days old barley lines PK-30108 and Haider-93 in gravel using NaCl concentrations (EC: 5 (Control), 7.5 and 15 dS m-1) in 25% Hoagland nutrients solution. Under increased salinity stress both the barley lines underwent significant decrease in shoot and root dry biomass, chlorophyll (a, b and total) and total sugars whereas a significant increase in total amino acids and NIRA was recorded. The reduction was significantly more pronounced at EC 15 dS m-1 than EC 7.5 dS m-1 and the control. The line PK-30108 proved significantly more salt tolerant than the cultivar Haider-93 in respect of shoot and root biomass up to EC 7.5 dS m-1 while both remained similar at EC 15 dS m-1. The NRA was found to be unaffected in line Haider 93 but was reduced significantly in line PK-30108 with elevating salinity levels. The parameters could be used as biochemical markers for investigating the elevated salinity effects on barley varieties

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