Is Crohn’s Creeping Fat an Adipose Tissue?
Olivier , Isabelle(co-premier auteur) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire) | Theodorou , Vassilia(collaborateur) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire) | VALET , Philippe(collaborateur) (INRA , Marseille (France). UMR 1260 Nutrition, Obésité et Risques Thrombotiques ) | Castan-Laurell , Isabelle(collaborateur) (Institut National de la Sante et de la Recherche Medicale, Toulouse(France). I2MR, Adipocyte secretions, Obesities and Associated Pathologies team) | Guillou , Hervé(collaborateur) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire) | Bertrand-Michel , Justine(collaborateur) ( Institut National de la Santé et de la Recherche Médicale, Toulouse(France). IFR150, INSERM U563,) | Cartier , Christel(collaborateur) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire) | Bezirard , Valerie(collaborateur) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire) | Ducroc , Robert(collaborateur) (Institut National de la Sante et de la Recherche Medicale, Paris(France). INSERM U773, Center of Biomedical Research Bichat-Beaujon, CRB3) | Segain , Jean-Pierre(collaborateur) (INRA , Nantes (France). UMR 1280 Physiologie des Adaptations Nutritionnelles) | Portier , Guillaume(collaborateur) (Purpan University Hospital, Toulouse(France). Department of Digestive Surgery) | Kirzin , Sylvain(collaborateur) (Purpan University Hospital, Toulouse(France). Department of Digestive Surgery) | Moreau , Jacques(collaborateur) (Rangueil University Hospital, Toulouse(France). Department of Digestive Surgery) | Duffas , Jean-Pierre(collaborateur) (Rangueil University Hospital, Toulouse(France). Department of Digestive Surgery) | Ferrier , Laurent(collaborateur) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire) | Eutamene , Hélène(auteur de correspondance) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire)
Background: In human pathology, the ‘‘creeping fat’’ (CF) ofthe mesentery is unique to Crohn’s disease (CD). CF is usuallyreferred to as an ectopic extension of mesenteric adipose tissue(MAT). However, since no animal model developing CF has everbeen established, very little is known about this type of fat-depotexpansion and its role in the development of the disease.Methods: We developed and standardized an experimental protocolin mice that reproducibly induces CF development when asevere colonic inflammation is obtained by intracolonic instillationof DNBS.Results: Macro-microscopic observations revealed a fattyappearance of CF. Yet when compared to MAT from the sameanimals, CF contains very little triglycerides, few adipocytes, andwe observed a very low expression and protein levels of both adiposemarkers (hormone-sensitive lipase, perilipin) and adipocytokines(leptin, adiponectin). The decreased expression of perilipinin CF was also observed by immunohistochemistry. Conversely,the expression of proinflammatory and fibrous markers (Pref-1)was much higher in CF than in MAT. These observations werefully consistent with those made on CF recovered from five CDpatients and compared with subcutaneous and mesenteric fat fromthe same patients.Conclusions: Altogether, this work reports an original experimentalmice model of CF. In this model we establish for the firsttime that CF only occurs in severe colonic inflammation andshows an inflammatory, fibrous but not an adipose pattern.
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