An expanded protein–protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach
2011
Marchadier , Elodie (INRA , VersaillesJouy-En-Josas (France). UMRUMRUR 131813191077 Institut Jean-Pierre BOURGINMICrobiologie de l'ALImentation au Service de la Santé HumaineUnité de recherche Mathématique, informatique et génome) | Carballido Y Lopez , Rut (INRA , Jouy-En-Josas (France). UMR 1319 MICrobiologie de l'ALImentation au Service de la Santé Humaine) | Brinster , Sophie (Institut National de la Santé et de la Recherche Médicale, Paris(France). Inst Cochin) | Fabret , Celine (Centre National de la Recherche Scientifique, Orsay(France). IGM) | Mervelet , Peggy (INRA , Jouy-En-Josas (France). UMR 1319 MICrobiologie de l'ALImentation au Service de la Santé Humaine) | Bessières , Philippe (INRA , Jouy-En-Josas (France). UR 1077 Mathématique, Informatique et Génome) | Noirot-Gros , Marie Francoise (INRA , Jouy-En-Josas (France). UMR 1319 MICrobiologie de l'ALImentation au Service de la Santé Humaine) | Fromion , Vincent(auteur de correspondance) (INRA , Jouy-En-Josas (France). UR 1077 Mathématique, Informatique et Génome) | Noirot , Philippe(auteur de correspondance) (INRA , Jouy-En-Josas (France). UMR 1319 MICrobiologie de l'ALImentation au Service de la Santé Humaine)
We have generated a protein-protein interaction network in Bacillus subtilis focused on several essential cellular processes such as cell division, cell responses to various stresses, the bacterial cytoskeleton, DNA replication and chromosome maintenance by careful application of the yeast two-hybrid approach. This network, composed of 793 interactions linking 287 proteins with an average connectivity of five interactions per protein, represents a valuable resource for future functional analyses. A striking feature of the network is a group of highly connected hubs (GoH) linking many different cellular processes. Most of the proteins of the GoH have unknown functions and are associated to the membrane. By the integration of available knowledge, in particular of transcriptome data sets, the GoH was decomposed into subgroups of party hubs corresponding to protein complexes or regulatory pathways expressed under different conditions. At a global level, the GoH might function as a very robust group of date hubs having partially redundant functions to integrate information from the different cellular pathways. Our analyses also provide a rational way to study the highly redundant functions of the GoH by a genetic approach.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Institut national de la recherche agronomique
