A rapid genotyping method for an obligate fungal pathogen, Puccinia striiformis f.sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping
Ali , Sajid (INRA , Thiverval-Grignon (France). UR 1290 BIOlogie GEstion des Risques en agriculture - Champignons Pathogènes des Plantes) | Gautier , Angelique (INRA , Thiverval-Grignon (France). UR 1290 BIOlogie GEstion des Risques en agriculture - Champignons Pathogènes des Plantes) | Leconte , Marc (INRA , Thiverval-Grignon (France). UR 1290 BIOlogie GEstion des Risques en agriculture - Champignons Pathogènes des Plantes) | Enjalbert , Jerome (INRA , Gif-Sur-Yvette (France). UMR 0320 Génétique Végétale) | Pope De Vallavieille , Claude (INRA , Thiverval-Grignon (France). UR 1290 BIOlogie GEstion des Risques en agriculture - Champignons Pathogènes des Plantes)
Puccinia striiformis f.sp. tritici (PST), an obligate fungal pathogen causing wheat yellow/stripe rust, a serious disease, has been used to understand the evolution of crop pathogen using molecular markers. However, numerous questions regarding its evolutionary history and recent migration routes still remains to be addressed, which need the genotyping of a large number of isolates, a process that is limited by both DNA extraction and genotyping methods. To address the two issues, we developed here a method for direct DNA extraction from infected leaves combined with optimized SSR multiplexing.
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