Comparative assessment of the intracellular survival of the Burkholderia pseudomallei bopC mutant
2013
Srinon, V., Mahidol University, Bangkok, Thailand | Muangman, S., Mahidol University, Bangkok, Thailand | Imyaem, N., Mahidol University, Bangkok, Thailand | Muangsombut, V., Mahidol University, Bangkok, Thailand | Adler, N.R.L., University of Leicester, Leicester, UK | Galyov, E.E., University of Leicester, Leicester, UK | Korbsrisate, S., Mahidol University, Bangkok, Thailand
Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei.
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