Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp
2013
Shin, S.W., Seoul National University, Seoul, Republic of Korea | Cha, S.B., Plant and Fisheries Quarantine and Inspection Agency, Anyang, Republic of Korea | Lee, W.J., Seoul National University, Seoul, Republic of Korea | Shin, M.K., Seoul National University, Seoul, Republic of Korea | Jung, M., Seoul National University, Seoul, Republic of Korea | Yoo, A., Seoul National University, Seoul, Republic of Korea | Jung, B.Y., Seoul National University, Seoul, Republic of Korea | Yoo, H.S., Seoul National University, Seoul, Republic of Korea
The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle (CT) of Salmonella spp. was 11.83 ± 0.78 while non-Salmonella spp. was 30.86 ± 1.19. Correlation coefficients of standard curves constructed using CT versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity (R2 = 0.993; slope = 3.563). Minimum level of detection with the method was 102 colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.
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