Comparison of Vitrification and Slow Freezing for the Cryopreservation of Chicken Primordial Germ Cell(Ogye)
2013
Kim, S.W., RDA, Namwon, Republic of Korea | Ko, Y.G., RDA, Namwon, Republic of Korea | Byun, M.J., RDA, Namwon, Republic of Korea | Do, Y.J., RDA, Namwon, Republic of Korea | Han, J.Y., Seoul National University, Seoul, Republic of Korea | Kim, D.H., RDA, Namwon, Republic of Korea | Seong, H.H., RDA, Namwon, Republic of Korea | Kim, H., RDA, Namwon, Republic of Korea
We sought to provide a method for freezing and preserving primordial germ cells, or an avian germ cell of a bird, as a material for developmental engineering or species preservation. The aim of this study was to compare the efficacy of slow freezing with a vitrification method for the cryopreservation of chicken primordial germ cells(PGCs). PGCs obtained from the germinal gonad of day 5.5-6 day(stage 28) cultured chick embryos, using the MACS method, were classified into two groups: slow freezing and vitrification. We examined the viability of PGCs after Cryopreservation. Four freezing methods were compared with each other, including the following: Method 1: The PGCs were frozen by a programmed freezer in a plastic straw, including 2.0M ethylene glycol(EG) as cryoprotective additive(slow freezing) Method 2: The PGCs were vitrified in a plastic straw, including 8.0 M EG, plus 7% polyvinylpyrrolidone(PVP)(rapid freezing). Method 3: The slow freezing was induced with a cryotube including 2.0 M EG Method 4: The PGCs were frozen in a cryotube including 10% dimethyl suloxide(DMSO)(rapid freezing). After freezing and thawing, survival rates of the frozen-thawed PGCs from Method 1 to 4were 76.4%, 70.6%, 80.5% and 78.1%(p0.05), respectively. The slow freezing(－80℃ programmed freezer) method may provide better survival rates of frozen-thawed PGCs than the vitrification method for the cryopreservation of PGCs. Therefore, these systems may contribute to the cryopreservation of a rare avian species.
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