Two-dimensional Electrophoresis of High Molecular Weight Glutenin Subunits in Korean Wheat Cultivars
2013
Lee, J.Y., RDA, Suwon, Republic of Korea | Park, C.S., Chonbuk National University, Jeonju, Republic of Korea | Kim, H.J., RDA, Suwon, Republic of Korea | Kim, J.H., RDA, Suwon, Republic of Korea | Kim, M.S., RDA, Suwon, Republic of Korea | Kim, Y.T., KRIBB, Daejeon, Republic of Korea | Kang, C.S., RDA, Suwon, Republic of Korea | Lim, S.H., RDA, Suwon, Republic of Korea | Ha, S.H., Kyung Hee University, Yongin, Republic of korea | Ahn, S.N., Chungnam National University, Daejeon, Republic of Korea | Kim, Y.M., RDA, Suwon, Republic of Korea
To evaluate qualitative and quantitative expression level of HMW-GS protein, we separated glutenin fractions and conducted two-dimensional electrophoresis (2DE) in 32 Korean wheat cultivars. The average spot numbers of HMW-GS in Korean wheat cultivars were 11.78 which included 1.31, 5.53 and 4.94 spots at Glu-A1, Glu-B1 and Glu-D1 loci, respectively. Cultivars harboring 1, 2 subunits had more spots than ones with null allele in Glu-A1 locus because Glu-B1 and Glu-D1 loci did not show difference in spot number. A large variation in the total spot number of HMW-GS was observed ranging from 7 (Dahong) to 18 (Jokyung). Relative expression level of Korean wheat cultivars when compared with the check accession Chinese Spring ranged from 24% (Eunpa) to 111% (Gobun) with an average of 44%. Korean wheat accessions could be classified into six groups based on the number of HMW-GS spots and quantification value of each spot.
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