Anti-inflammatory Activity of an Ethanol Extract of Laminaria japonica Root on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells
2014
Kang, B.K., Pukyong National Univerity, Busan, Republic of Korea | Kim, K.B.W.R., Pukyong National Univerity, Busan, Republic of Korea | Kim, M.J., Pukyong National Univerity, Busan, Republic of Korea | Bark, S.W., Pukyong National Univerity, Busan, Republic of Korea | Pak, W.M., Pukyong National Univerity, Busan, Republic of Korea | Kim, B.R., Pukyong National Univerity, Busan, Republic of Korea | Ahn, N.K., Pukyong National Univerity, Busan, Republic of Korea | Choi, Y.U., Pukyong National Univerity, Busan, Republic of Korea | Ahn, D.H., Pukyong National Univerity, Busan, Republic of Korea
Laminaria japonica roots have not been used practically in Korea. In this study, in order to promote the use of these by-products, the anti-inflammatory activity of an ethanol extract of Laminaria japonica root (LJREE) was investigated using a lipopolysaccharide (LPS) to induce an inflammatory response in RAW 264.7 cells. To examine the potential anti-inflammatory effects of LJREE, levels of nitric oxide (NO) and pro-inflammatory cytokines, such as interleukin-6 (IL-6), tumor necrosis factor-¥� (TNF-¥�), and interleukin-1¥� (IL-1¥�), and cell proliferation were measured. We found that NO levels decreased in a dose-dependent manner, the production of IL-6, TNF-¥�, and IL-1¥� was suppressed, and the production of IL-1¥� was inhibited over 30% after treatment with 100 ¥�g/mL LJREE. In conclusion, the proliferation of RAW 264.7 cells, measured by MTT assay, confirmed that LJREE may have significant effects on inflammatory factors without any cytotoxicity, making it a potential anti-inflammatory agent.
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