Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen Spermatozoa
Kim, M.S., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Choi, A., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, C.L., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, D., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Seong, H.H., National Institute of Animal Science, RDA, Namwon, Republic of Korea | Kim, S.W., National Institute of Animal Science, RDA, Namwon, Republic of Korea
Cryopreservation of germ cells from genetically proven animals could be a source of restoration tools from the risk of extinction or disappearance of wanted characteristics. Using frozen semen, the genetic gains of Korean native cattle have been increased greatly for 70 years. The preservation of genetic resources as a form of frozen semen straw has limited availability due to the numbers. To circumvent this weakness of frozen semen, we tested two re-freezing methods with different initial thawing temperatures using frozen Korean proven semen and rare breed semen from albino, black and chikso breeders. It has been known that human sperm could resist to cryo-damages by repeated freeze-thaw cycles, but not for Korean proven bulls number (KPN) or for rare breeds. Total 7 frozen semem from brindled(2), black(1), Korean Albino(2) and KPN(1) bulls were used for our research. After thawing straws under 5 degree Celsius/2min or 37 degree Celsius/40sec with low temperature water bath and thermo jug, spermatozoa were re-diluted with triladyl diluents after first thawing and re-frozen. Sperm motilities were compared between animals and treated groups after re-thawing. Mean values of motility and viability of refrozen/thawed sperm for expansion of the number of straws were significantly higher in 5°C than in 37 degree Celsius(P less than less than 0.05). However, the activity of viable sperm thawed at 5 degree Celsius was significantly decreased after first and second thawing. It is estimated that re-freezing of frozen semen from rare Korean native cattle is possible with resistant properties of survived spermatozoa.
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