Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature
2018
Guoliang Yuan, Beijing Academy of Agriculture and Forestry, Beijing, China | Yukun Wang, Beijing Academy of Agriculture and Forestry, Beijing, China | Shaohua Yuan, Beijing Academy of Agriculture and Forestry, Beijing, China | Peng Wang, Beijing Academy of Agriculture and Forestry, Beijing, China | Wenjing Duan, Beijing Academy of Agriculture and Forestry, Beijing, China | Jianfang Bai, Beijing Academy of Agriculture and Forestry, Beijing, China | Hui Sun, Beijing Academy of Agriculture and Forestry, Beijing, China | Na Wang, Beijing Academy of Agriculture and Forestry, Beijing, China | Fengting Zhang, Beijing Academy of Agriculture and Forestry, Beijing, China | Liping Zhang, Beijing Academy of Agriculture and Forestry, Beijing, China | Changping Zhao, Beijing Academy of Agriculture and Forestry, Beijing, China
Thermosensitive male sterility plays an important role in wheat fertility and production. As a key enzyme for chlorophyll degradation, pheophorbide a oxygenase (PaO) can suppress cell death in plants. We cloned the wheat gene TaPaO1 from the thermosensitive genetic male sterile (TGMS) line BS366; it encodes a typical PaO protein, containing a conserved Rieske [2Fe-2S] iron-sulphur motif, a mononuclear non-heme iron-binding motif, and a C-terminal CxxC motif. TaPaO1 was expressed in all tissues and was upregulated during the meiosis stage of BS366 anthers under low temperature. Subcellular localization of TaPaO1 specifically labelled the surrounding of chloroplasts. TaPaO1 regulated by RD29A promoter which responded to low temperature led to pollen sterility in transgenic tobacco. Expression analysis showed that TaPaO1 exhibited a higher level of expression in the anther than in other tissues in transgenic tobacco plants during low temperature treatment. We propose that the higher senescence-related activity of TaPaO1 may lead to the cell death of anthers, which happens at an early developmental stage under low temperature. These results provide new insights into the function of PaO during the early developmental stage of anthers. PaO is closely related to cell death regardless of whether it exhibits increased activity or inactive.
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