A single step method for purification of sulfated oligosaccharides
Garénaux, Estelle | Yu, S.-Y. | Florea, Doina | Strecker, Gerard | Khoo, Kay-Hooi | Guerardel, Yann
Purifying and analysing sulfated oligosaccharides by mass spectrometry often constitutes a challenge. We present here a single step method to isolate sulfated compounds from a complex mixture of neutral and acidic oligosaccharide-alditols. The strategy relies on the exclusion of sulfated molecules from strong cation exchange resin. By testing a wide range of reduced mucin type O-glycans isolated from different biological sources, we demonstrate that this method permits, without prior chemical modification, the specific purification of sulfate-containing oligosaccharides present in any quantity from very complex mixtures of molecules.
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Эту запись предоставил National Institute for Agricultural Research