Role of protective agents on the viability of probiotic Lactobacillus plantarum during freeze drying and subsequent storage

Charoen, R.; Kerdwan, N.; Tipkanon, S.; Savedboworn, W.; Sakorn, A.; Pattayakorn, K.

Role of protective agents on the viability of probiotic Lactobacillus plantarum during freeze drying and subsequent storage

2017

The aim of this study was to determine the effect of various protective agents on the survivalof probiotic Lactobacillus plantarum TISTR 2075 grown in Plai Ngahm Prachin Buri riceextract during freeze drying and subsequent storage. A combination of protein-trehalose (Prot+ Tre) and protein-maltodextrin (Prot + MD) significant (P<0.05) improved the viability of thestrain after freeze drying with the survival rate of 98.13 and 97.58%, respectively. Among allprotective agents tested, Prot + Tre was found to maintain high degrees of viable cell numberwith the lowest specific rate of cell death (k) of 7.45 x 10-4 and 1.79 x 10-2 day-1 after storage at4oC for 168 days and 30oC for 84 days, respectively. Additionally, the accelerated storage testsusing accelerated temperatures of 40, 50, 60 and 70oC were used to develop a model system inorder to estimate the viability of freeze-dried probiotic L. plantarum TISTR 2075 in differentprotective agents for long-term storage. It was concluded that accelerated storage testing is auseful technique with certain predictability in this study.

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