Investigation of the 18S rRNA gene sequence of Hepatozoon canis detected in Indian dogs

Bal, M. S.; Sumbria, Deepak; Kaur, Paramjit; Singla, L. D.; Mandhotra, Ajay

Investigation of the 18S rRNA gene sequence of Hepatozoon canis detected in Indian dogs

2017

Bal, M. S. | Sumbria, Deepak | Kaur, Paramjit | Singla, L. D. | Mandhotra, Ajay

Canine hepatozoonosis isa growing tick-borne disease in Punjab.Two canine hepatozoonosis cases, oneclinical and one subclinical, in Punjabwere analyzed by PCR targeting 18S rRNAgene (666 bp). After sequence analysisof the PCR products, both of them werefound almost identical to each other andwere closely related to the Hepatozooncanis strain found in Saint kitts and Nevisand Brazil with 100% (442/442) and 99%(440/442) nucleotide identity respectively.Isolates from Malta and Philippines ofH. canis were distantly related to IndianH. canis with 437/442 and 436/442 matchidentities. These results suggest that H.canis detected in north Indian dogs mighthave closer ancestral relationship with SaintKitts and Nevis followed by Brazil strain.This is the first molecular characterizationof Hepatozoon from Punjab, India.

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