Effect of Different Extenders on Awassi Rams Frozen Semen Fertility.
2016
D. Tainturier | M. R. Al- Merestani | M. Moussa | M. Bashawat
The research was carried out at Izra’a research station (Arab Center for the Studies of Arid Zones and Dry lands / ACSAD) during the mating season of 2014 to evaluate the fertilizing ability of Awassi ram’s frozen semen diluted in three extenders, and to test the oxytocin treatment effectiveness before cervical artificial insemination (CAI).Ninety Awassi ewes were randomly divided into three equal main-groups to test the extender impact on semen quality; each main group was divided into two equal subgroups to test the oxytocin impact. Ewes in synchronized-estrus (through vaginal sponges) of each main group were inseminated using frozen-thawed semen diluted in one of the following extenders: standard (AndroMed®), egg yolk (20%) adds 25 mM/ml proline (EY+25P), and low-density lipoprotein (8%)adds 25 mM/ml proline (LDL+25P) solutions. Ewes of three subgroups were intravenous injected with oxytocin (20 IU) 10 minutes before CAI.Results showed positive effect of (LDL+25P) extender in fertilization and lambing rates (62.06±0.93 and 41.38±0.91 %, respectively) compared with two extenders, where (56.67±0.91 and 40±0.90 %) and (48.28±0.93 and 31.03±0.91 %) recorded, respectively in (AndroMed®) and (EY+25P) extender-groups.Fertilization and lambing rates were negatively influenced by oxytocin treatment, they decreased from 65.12±0.76 and 44.19±0.75 % in non-treated ewes to 46.67±0.74 and 31.12±0.73 % in treated ewes, respectively. In conclusion, the use of LDL fractions with proline increased significantly fertilization and lambing rates, while the effect of oxytocin treatment was negative in both traits.
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