Molecular detection of pathogenic Leptospira spp. in urban rodents from wet markets in northeast Malaysia
Intan Noor Aina Kamaruzaman (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Muhamad Aiman Mohd Mokhtar (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Hong Wei Ting (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Yong Kai Yuan (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Azim Wafiy Gulam Shah (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Tan Wan Loong (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Nurshahirah Shaharulnizim (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Mohd Farhan Hanif Reduan (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Fathin Faahimaah Abdul Hamid (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Nur Amalina Noralidin (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Nur Athirah Abdul Manaf (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Che Wan Salma Che Wan Zalati (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia) | Loong Shih-Keng (Tropical Infectious Diseases Research & Education Centre, Universiti Malaya, Kuala Lumpur, Malaysia) | Simon Clegg (School of Life Sciences, University of Lincoln, Brayford Pool Lincoln, United Kingdom) | Luqman Abu-Bakar (Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bharu, Malaysia.)
Objective: This short study describes the occurrence of pathogenic Leptospira spp. in two major wet markets in Kota Bharu, Kelantan, Malaysia. Materials and Methods: 30 rodents (20 rats and 10 shrews) were caught in 2 wet markets, and a postmortem was performed to extract both kidneys. Molecular diagnosis via polymerase chain reaction (PCR) was conducted to detect leptospiral DNA using universal and pathogenic Leptospira primers, respectively. Results: The results showed that 20/28 (72%) rat samples were detected positive for Leptospira spp, and all shrews were negative. Further sequencing analysis identified L. interrogans and L. borgpetersenii as the most frequently Leptospirosis species from kidney samples. Conclusions: The presented study here sheds light on the presence of pathogenic leptospires har¬boring the rat population in both wet markets in Kelantan, which presents a great public health risk to wet market workers and visitors. [J Adv Vet Anim Res 2022; 9(2.000): 275-281]
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