Systemic infection by Spencermartinsiella sp. in a Nile crocodile (Crocodylus niloticus)

Tatiane Furtado de Carvalho; Herlandes Penha Tinoco; Marcelo Cordeiro Campos Malta; Angela Tinoco Pessanha; Thelma Tirone Silvério Matos; Cláudia Emanuela Rocha; Priscilla Parreira Rocha Gomes; Luize Néli Nunes Garcia; Daniella Noronha Frois; Marianna de Paula Martins Pereira; Susana Johann; Carlos Augusto Rosa; Tatiane Alves da Paixão; Renato Lima Santos

Systemic infection by Spencermartinsiella sp. in a Nile crocodile (Crocodylus niloticus)

2017

A male adult crocodile (Crocodylus niloticus) was diagnosed with systemic yeast infection. Histologically, there were extensive areas of necrosis in the lung, which were associated with a diffuse severe lympho-plasmo-histiocytic inflammatory infiltrate, with numerous multinucleated giant cells, and myriads of intralesional pseudo-hyphae and yeast like organisms within distended foveolae. Necrotic foci were also observed in the mucosa of the digestive tract, trachea, tunica intima of arteries, liver, and heart, with a marked inflammatory lympho-histiocytic infiltrate, with large numbers of epithelioid macrophages and giant cells, and intralesional and intravascular pseudo-hyphae and yeast-like organisms. Oval yeast structures with 4 to 6 μm in diameter and 5 to 8 μm thick paralleled-wall pseudo-hyphae were observed in PAS or GMS stained sections. PCR with DNA template extracted from paraffin embedded tissues amplified the D1/D2 domains of the large subunit rRNA gene, which was sequenced and found to be identical to sequences of a new species, isolated from rotting wood in Brazil, of the genus Spencermartinsiella, which its closest relative is Spencermartinsiella cellulosicola.

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