4-hydroxy-2-nonenal as a marker of the oxidative stress in brains of  dogs with canine distemper

Érica Almeida Viscone; Lígia Assunção Oliveira; Alexandra Ariadine Bittencourt Gonçalves Pereira; Márcio Botelho  Castro; Paulo Eduardo  Brandão; Sueli Akemi  Taniwaki; Aline Santana da Hora; Márcio de Barros  Bandarra

4-hydroxy-2-nonenal as a marker of the oxidative stress in brains of dogs with canine distemper

2022

Canine Distemper is a disease caused by Canine morbillivirus (CM), a pantropic virus that can affect the central nervous system (CNS), causing demyelination. However, the pathogenesis of this lesion remains to be clarified. Brain samples of 14 naturally infected dogs by CM were analyzed to evaluate the presence of oxidative stress and demyelination. RT-PCR assay was performed to confirm a diagnosis of canine distemper in the brain, immunohistochemistry anti-CM was used to localize the viral proteins in the tissue, and anti-4-hydroxy-2 nonenal (4-HNE) was a marker of a product of lipid peroxidation. The results showed the presence of viral proteins in the demyelinated area with the presence of 4-HNE. Our results suggest that the CM virus infection causes oxidative stress leading to lipid peroxidation, which causes tissue damage and demyelination. In conclusion, oxidative stress plays a significant role in canine distemper pathogenesis in the CNS.

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