The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence.
2020
Laure N A Ries | Lakhansing Pardeshi | Zhiqiang Dong | Kaeling Tan | Jacob L Steenwyk | Ana Cristina Colabardini | Jaire A Ferreira Filho | Patricia A de Castro | Lilian P Silva | Nycolas W Preite | Fausto Almeida | Leandro J de Assis | Renato A C Dos Santos | Paul Bowyer | Michael Bromley | Rebecca A Owens | Sean Doyle | Marilene Demasi | Diego C R Hernández | Luís Eduardo S Netto | Monica T Pupo | Antonis Rokas | Flavio V Loures | Koon H Wong | Gustavo H Goldman
Aspergillus fumigatus is an opportunistic fungal pathogen that secretes an array of immune-modulatory molecules, including secondary metabolites (SMs), which contribute to enhancing fungal fitness and growth within the mammalian host. Gliotoxin (GT) is a SM that interferes with the function and recruitment of innate immune cells, which are essential for eliminating A. fumigatus during invasive infections. We identified a C6 Zn cluster-type transcription factor (TF), subsequently named RglT, important for A. fumigatus oxidative stress resistance, GT biosynthesis and self-protection. RglT regulates the expression of several gli genes of the GT biosynthetic gene cluster, including the oxidoreductase-encoding gene gliT, by directly binding to their respective promoter regions. Subsequently, RglT was shown to be important for virulence in a chemotherapeutic murine model of invasive pulmonary aspergillosis (IPA). Homologues of RglT and GliT are present in eurotiomycete and sordariomycete fungi, including the non-GT-producing fungus A. nidulans, where a conservation of function was described. Phylogenetically informed model testing led to an evolutionary scenario in which the GliT-based resistance mechanism is ancestral and RglT-mediated regulation of GliT occurred subsequently. In conclusion, this work describes the function of a previously uncharacterised TF in oxidative stress resistance, GT biosynthesis and self-protection in both GT-producing and non-producing Aspergillus species.
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