Collection of Macaca fascicularis cDNAs derived from bone marrow, kidney, liver, pancreas, spleen, and thymus

Kameoka Yosuke; Kusuda Jun; Terao Keiji; Sugano Sumio; Suzuki Yutaka; Tanuma Reiko; Hirata Makoto; Osada Naoki; Hashimoto Katsuyuki; Takahashi Ichiro

Collection of Macaca fascicularis cDNAs derived from bone marrow, kidney, liver, pancreas, spleen, and thymus

2009

Abstract Background Consolidating transcriptome data of non-human primates is essential to annotate primate genome sequences, and will facilitate research using non-human primates in the genomic era. Macaca fascicularis is a macaque monkey that is commonly used for biomedical and ecological research. Findings We constructed cDNA libraries of Macaca fascicularis, derived from tissues obtained from bone marrow, liver, pancreas, spleen, and thymus of a young male, and kidney of a young female. In total, 5'-end sequences of 56,856 clones were determined. Including the previously established cDNA libraries from brain and testis, we have isolated 112,587 cDNAs of Macaca fascicularis, which correspond to 56% of the curated human reference genes. Conclusion These sequences were deposited in the public sequence database as well as in-house macaque genome database <url>http://genebank.nibio.go.jp/qfbase/</url>. These data will become valuable resources for identifying functional parts of the genome of macaque monkeys in future studies.

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