Micropropagation of five species of Campanula growing on the territory of Botanical Garden named prof. B.M. Kozo-Polyansky of Voronezh State University
2016
O. A. Zemlyanukhina | V. N. Kalaev | V. S. Voronina | O. S. Korneeva
The development of evidence-based methods of reproduction of protected and highly decorative species that are in high demand for use in landscaping, as well as the establishment of gene pools of species and varieties of plants in actively growing collection corresponds to the main direction of the state coordination program for the development of biotechnology in the Russian Federation until 2020 «BIO 2020». The on-standing research developed and optimized the conditions for obtaining actively proliferating cultures of 5 disappearing species; Campanula rotundifolia L., Campanula persicifolia L., Campanula trachelium L., Campanula rapunculoides L., Campanula glomerata L. Optimized conditions of rhizogenesis. The effect of the different sterilization and antibiotics concentration (kanamycin, benzyl penicillin, gentamicin, klaforan) on the growth and development of adventitious plants has been studied. Solutions of antibiotics for sterilization in addition to chlorine-containing agent («Belizna») and mercury (thimerosal) are needed. The possible negative effects of the addition of antibiotics in incubation solution lead to increasing of somaclonal variation, neсrosis, disturbance of chlorine synthesis («fatal lack of chlorophyll»), slow down the growth of adventitious shoots, and others. It is proposed LED lighting culture in a rack 16 hour period lighting LED strip 12 V power 4.5 W/m (3 LED 10 cm tape), general illumination of 2500–3000 lux. Each shelf is added with red tape photodiodes. The results allow, if necessary, to obtain plantation quantity of these kinds of Campanula in a short time.
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