Effect of Di-ethyl aminoethyl (DEAE) Dextran on the infectivity titre of sheep pox virus in-vitro and in-vivo

Olfat E. Nakhla; Namaa A. Mohamed; Manal Awad; Mervat M. Ali

Effect of Di-ethyl aminoethyl (DEAE) Dextran on the infectivity titre of sheep pox virus in-vitro and in-vivo

2010

Olfat E. Nakhla | Namaa A. Mohamed | Manal Awad | Mervat M. Ali

The effect of diethyl aminoethyl (DEAE) dextran on the infectivity titre of sheep pox virus (SPV) was studied with different concentrations (25, 50, 75, 100 μg/ml) of DEAE-dextran on Vero cell culture. It was found that 25 and 50μg/ml were not toxic. The same concentrations were used with sheep pox virus inoculum showing that the best virus titre (106.3 TCID50/ml) reached with the use of 25μg/ml DEAE-dextran after 10 passages. The enhanced viral fluid was tested in-vivo, by vaccination of susceptible lambs and challenge of them with the virulent sheep pox virus. These lambs showed complete protection against the disease. The SP neutralizing antibody indices (NI) were estimated in the collected serum samples post vaccination and challenge; confirmed that 25μg of DEAE-dextran/ml virus-inoculum induced an increase in neutralizing antibodies in comparison with those induced by currently used sheep pox vaccine.

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