Studies on recent IBD virus field variant isolates:Genomic identification and differentiation using RT-PCR-RFLP

M. M. Amer; S. A. Nassif

Studies on recent IBD virus field variant isolates:Genomic identification and differentiation using RT-PCR-RFLP

2005

M. M. Amer | S. A. Nassif

In the present study, 3 pooled proventricular homogenates were collected from 3broiler flocks, of chicken 15 to 30 days old, from Monofia Governorate. The 3 flockswere suffered from low growth rate, poor feed conversion rate, uneven growth andincreased mortalities. Necropsy of dead chickens revealed proventriculitis withincreased proventriculus size. IBD viral antigen was detected in the pooledproventricular homogenate of each flock by AGPT using reference antibodies againstIBDV and RT-PCR technique. No other viruses were detected; such as Reo virus,CAV, NDV, IBV and ALV-J. Further characterization of the IBDV isolates wereconducted by RFLP assay on PCR products using MboI and BstOI restrictionenzymes. Results demonstrate that the 3 IBDV isolates are identical in their RFLPpattern and related to the Del/E variant strain of IBDV.

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