Preparation of diagnostic lysate antigens for rapid evaluation of local entero-3 vaccine

A. A., EI-Kholy; Amal M. EI-Sawah; M. S., Wassel; S. M., Zeidan; A. M., Daoud

Preparation of diagnostic lysate antigens for rapid evaluation of local entero-3 vaccine

2005

A. A., EI-Kholy | Amal M. EI-Sawah | M. S., Wassel | S. M., Zeidan | A. M., Daoud

Studies were conducted to determine the utility of lysate antigens for rapidevaluation of the local entero-3 vaccine, antigens were prepared from cell culturesinfected with bovine rota virus (BRV) and bovine corona virus (BCV) as well asfrom Enterotoxigenic E. coli strain K99. Prepared antigens were tested with fieldserum samples collected from both late pregnant entero-3 vaccinated cows and their offsprings using different serological assays including: microagglutination test,indirect ELISA and immunofluorescent antibody technique. Results of this endeavorwere correlated to that of the standard virus neutralization test. The locally preparedantigens were proved useful for vaccine evaluation. Moreover, these antigens arerecommended for both detection and assessment post vaccination or post infection of sero-conversion against BRV, BCV and E. coli.

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