Camellia nitidissima Chi extract promotes adult hippocampal neurogenesis and attenuates chronic corticosterone-induced depressive behaviours through regulating Akt/GSK3β/CREB signaling pathway
2022
Bun Tsoi | Chong Gao | Shenyu Yan | Qiaohui Du | Hua Yu | Peng Li | Jiagang Deng | Jiangang Shen
Hormonal imbalance causes depressive behaviours in chronic mental disorders. The use of natural products to relieve depressive symptoms is getting attention due to its minimal side effects and multiple health benefits. The present study aims to test the hypothesis that Camellia nitidissima Chi leave extract (CNC) could have antidepressant effects and whose underlying mechanisms could be related to promote hippocampal neurogenesis. Firstly, we conducted quality control study and identified 17 active compounds in the CNC extract. Then, we performed a series of behaviour tests to evaluate the antidepressant effects in a chronic corticosterone (CORT)-induced depressive mouse model. CNC extract significantly ameliorated CORT-induced depressive behaviours, whose effects were similar to sertraline. Interestingly, CNC extract decreased the levels of CORT and ACTH in the plasma and increased 5-HT in plasma and hippocampus. CNC extract promoted adult hippocampal neurogenesis in the CORT-treated mice in vivo. The neurogenic effects of CNC were also confirmed in primary cultured hippocampal neurons and hESCs with CORT challenge in vitro. Furthermore, CNC enhanced the phosphorylation of Akt, GSK3β and CREB in the hippocampus of CORT-treated mice in vivo as well as CORT-treated PC12 cells in vitro. Co-treatment of wortmannin abolished the effects of CNC. Taken together, CNC could be an effective functional food to stimulate hippocampal neurogenesis for anti-depressant treatment. The neurogenic mechanisms could be related to regulating hypothalamic–pituitaryadrenal axis and activating Akt/GSK3β/CREB signaling pathway.
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