Diagnosis of paratuberculosis by microbiological culture in Veterinary Research Institute from 2001 to 2018

Mohammad Fhitri S.; Khoo E.; Saifu Nazri R.; Siti Norhanani R.; Roseliza R.; Norazariyah M. N.; Normah M. A.; Faizah Hanim M. S.

Diagnosis of paratuberculosis by microbiological culture in Veterinary Research Institute from 2001 to 2018

2019

Paratuberculosis (or Johne’s disease) is one of the most economically important diseases in the livestock industry. The disease is caused by Mycobacterium avium subsp. paratuberculosis and ischaracterised by a chronic progressively wasting syndrome. To date, no treatment is available for the disease and it invariably leads to the death of the infected animal. At present, microbiology culture is the gold standard diagnostic procedure to diagnose the disease. Both culture and serological methods are difficult and challenging. This paper reports the prevalence of Johne’s disease in ruminants based on microbiological culture submitted to Veterinary Research Institute, Ipoh from year 2001 to 2018. Throughout the 17 years, only 168 suspected Johne’s infection samples were received for culturing, including faeces and rectal pinch which were inoculated on selective mycobactin egg yolk media. Two positive cases of bovine, in the states of Sabah and Kedah, were recorded in year 2001 and 2018 respectively. Even though most of the microbiological culture were negative for M. paratuberculosis, the presence of the disease could not be overlooked. Due to the challenges in microbiological culture for diagnosis of the disease, the use of molecular techniques in combination with suitable serological methods is recommended in order to improve diagnosis of paratuberculosis in the laboratory.

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