The effect of two pre-treatment methods on biogas production potential from cow manure
2019
Nazaitulshila Rasit | Zulfah Zulkifli | Noor Azrimi Umor | Shahrul Ismail
Hydrolysis process is considered as a rate limiting step in anaerobic digestion (AD) of lignocellulosic material in cow manure (CM). Due to the negative effects of lignin in lignocellulosic material, only about 30 % of this material can be converted to biogas. To accelerate the hydrolysis process and increase the biogas production, pretreatment of CM is needed. Two different pretreatment methods, biological and physical, were evaluated. Four sets of anaerobic digestion assays were carried out, with a working volume of 500 mL at 35 ± 2 °C and 120 rpm. The first and second sets of digestion were classified under physical pretreatment which contained dry grinded (DG) and fresh blended (FB) pretreated samples. The third set of fermentation involved biological pretreatment with the addition of A. Fumigatus SK1, while the last set contained Trichoderma sp. These four pretreated samples were compared to the untreated CM. Several analyses were subjected to determine the biomethane potential (BMP) of CM. The highest BMP was observed in DG pretreatment (P<0.05) which was 0.27, followed by 0.17, 0.02 and 0.01 LCH4-STP g VS-1. These results were obtained after the samples were pretreated with FB and added with pretreated Trichoderma sp. and A. Fumigatus SK1, respectively. These results collectively suggested that DG pretreatment could be a promising pretreatment method for the higher methane production in the anaerobic mono-digestion process.
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