Validity of a commercial kit for detection of antibodies in bovine serum in an endemic area for fasciolosis

Simões,Aline Nunes; Almeida,Sayanne Luns Hatum de; Braga,Áquila Flávia da Rocha; Martins,Isabella Vilhena Freire; Donatele,Dirlei Molinari; Barioni,Graziela

Validity of a commercial kit for detection of antibodies in bovine serum in an endemic area for fasciolosis

2017

Abstract Fasciolosis is caused by Fasciola hepatica that affects the bile ducts and liver parenchyma of ruminants, which can result in economic loss. This study aimed to carry out the validity of the commercial kit ELISA® indirect front of the simple fecal sedimentation test used as the standard. 143 samples were collected blood and feces of cattle from Jerome, south of the Espírito Santo. Serum samples were left at -80 °C and used to perform the ELISA kit IDEXX®. All animals to stool examinations were also positive to the ELISA (22) and negative samples to test stool (121), 52 animals reacted positively against the antibody research. The frequency of fasciolosis was 15.4% in the stool examinations and 51.8% by ELISA. The validity was calculated by sensitivity (100%), specificity (57%), positive predictive value (29%) and negative predictive value (100%), and the correlation between the tests calculated using the kappa index of 0.35. The better sensitivity of the ELISA commercial kit should not be a separately evaluated, since the cost benefit and the technical facility must be considered.

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