Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study

Katrin Hufnagel; Bernice Hoenderboom; Christoph Harmel; Juliane K. Rohland; Birgit H.B. van Benthem; Servaas A. Morré; Tim Waterboer

Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study

2019

Chlamydia trachomatis (Ct) whole-proteome microarrays were utilized to identify antibody patterns associated with infection: pelvic inflammatory disease (PID), tubal factor infertility, chronic pelvic pain (CPP) and ectopic pregnancy in a subsample of the Netherlands Chlamydia cohort study. Serum pools were analyzed on whole-proteome arrays. The 121 most reactive antigens identified during whole-proteome arrays were selected for further analysis with minimized microarrays that allowed for single sera analysis. From the 232 single sera: 145 (62.5%) serum samples were reactive for at least one antigen. To discriminate between positive and negative serum samples: we created a panel of in total 18 antigens which identified 96% of all microarray positive samples. Antigens CT_858: CT_813 and CT_142 were most reactive. Comparison of antibody reactivity&rsquo:s among women with and without Ct related sequelae revealed that the reactivity of CT_813 and CT_142 was less common among women with PID compared to women without (29.0% versus 58.6%, p = 0.005 and 25.8% versus 50.6%, p = 0.017 respectively). CT_858 was less common among CPP cases compared to controls (33.3% versus 58.6: p = 0.028). Using a whole-proteome array to select antigens for minimized arrays allows for the identification of novel informative antigens as general infection markers or disease associated antigens

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