Linkage among isozyme, RFLP and RAPD markers in Vicia faba

Torres, A.M. . Dept. de Mejora y Agronomia; Weeden, N.F.; Martin, A.

Linkage among isozyme, RFLP and RAPD markers in Vicia faba

1993

Torres, A.M. (Centro de Investigacion y Desarollo Agrario, Cordoba (Spain). Dept. de Mejora y Agronomia) | Weeden, N.F. | Martin, A.

Segregating allozyme and DNA polymorphisms were used to construct a preliminary linkage map for faba bean. Two F(2) populations were analyzed, the most informative of which was segregating for 66 markers. 11 independently assorting linkage groups were identified in this population. One of the groups contained the 45s ribosomal array and could be assigned to the large metacentric chromosome 1 on which the nucleolar organizer region is located. This linkage group also contained 2 isozyme loci, Est and Tpi-p, suggesting that it may share some homology with chromosome 4 of garden pea on which 3 similar markers are syntenic. Additional aspects of the map and the extent of coverage of the total nuclear genome are discussed.

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