The Tn5 bleomycin resistance gene confers improved survival and growth advantage on Escherichia coli
1994
Blot, M. (Biozentrum, Basel (Switzerland). Dept. of Microbiology) | Hauer, B. | Monnet, G.
The bleomycin resistance gene (ble) of transposon Tn 5 is known to decrease the death rate of Escherichia coli during stationary phase. Bleomycin is a DNA-damaging agent and bleomycin resistance is produced by improved DNA repair which also requires the host genes aidC and polA coding, respectively, for an alkylation-inducible gene product and DNA polymerase 1. In the absence of the drug, this DNA repair system is believed to cause the slower death rate of bleomycin-resistant bacteria. In this study, the effect of ble and aidC genes on the viability of bacteria and their growth rate in chemostat competitions was studied. The results indicate, that bleomycin-resistant bacteria display greater fitness under these conditions. Another beneficial effect of transposon Tn5 had been previously attributed to the insertion sequence IS50R. It was not possible to reproduce this result with IS50R, however, the complete transposon was beneficial under similar conditions. Moreover, the Tn5 fitness effect was shown to be aidC-dependent. The ble gene was discovered after the fitness effect of IS50R had been established; it has not previously been considered to mediate the beneficial effect of Tn5. This possibility is discussed based on the molecular mechanism of bleomycin resistance.
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Эту запись предоставил ZB MED Nutrition. Environment. Agriculture
