A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses
2008
Abolnik, C.(ARC-Onderstepoort Veterinary Institute)
A Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (H0). The assay was used to pathotype H5-type viruses detected by rRT-PCR in ostrich tracheal swabs collected during the 2006 HPAI H5N2 outbreak in the Western Cape Province.
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Библиографическая информация
Onderstepoort Journal of Veterinary Research
Том
75
ISSN
0030-2465
Издатель
ARC-Onderstepoort Veterinary Institute - Agricultural Research Council
Другие темы
Fret; H5n2; Pathotyping; Real-time reverse transcription pcr
Язык
Английский
Тип
Journal Article; Journal Part
2023-09-25
AGRIS AP
Поставщик данных
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