Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

Villa-Mancera, Abel; Molina-Mendoza, Pedro; Hernández-Guzmán, Karina; Olivares-Pérez, Jaime; Sarracent-Pérez, Jorge; Zumaquero-Ríos, José

Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

2016

Villa-Mancera, Abel(Laboratorio de Biotecnología Agropecuaria y Biología Molecular, Facultad de Medicina Veterinaria y Zootecnia) | Molina-Mendoza, Pedro(Laboratorio de Biotecnología Agropecuaria y Biología Molecular, Facultad de Medicina Veterinaria y Zootecnia) | Hernández-Guzmán, Karina(Laboratorio de Biotecnología Agropecuaria y Biología Molecular, Facultad de Medicina Veterinaria y Zootecnia) | Olivares-Pérez, Jaime(Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero) | Sarracent-Pérez, Jorge(Laboratorio de Hibridomas, Instituto de Medicina Tropical Pedro Kourí) | Zumaquero-Ríos, José(Laboratorio de Parásitos y Vectores, Escuela de Biología)

The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P<0.01) for low seropositivity (r=0.93) and medium seropositivity (r=0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

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