A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus

Tseng, Chun-Hsien; Tsai, Hsiang-Jung; Chang, Chung-Ming

A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus

2014

Tseng, Chun-Hsien(Animal Health Research Institute, Danshui, Taipei 25158) | Tsai, Hsiang-Jung(Animal Health Research Institute, Danshui, Taipei 25158) | Chang, Chung-Ming(Research Center for Emerging Viral Infections, Department of Medical Biotechnology and Laboratory Science)

Introduction. The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. Method. This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. Result and Conclusion. This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

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