Development and utility of a tri-primer PCR for simultaneous detection of cattle, sheep and pig in meat products

Pingya, W.; Feng, G..; Jin, Z.; Yuanyuan, L.; Chaobo, X.

Development and utility of a tri-primer PCR for simultaneous detection of cattle, sheep and pig in meat products

2017

Pingya, W. | Feng, G.. | Jin, Z. | Yuanyuan, L. | Chaobo, X.

In this study, a tri-primer multiplex PCR method was developed for simultaneous detection of pig, sheep and cattle DNA from meat products. Three primers were designed in mitochondrial DNA region, included one universal downstream primer and two upstream specific primers. PCR reactions generated 570 bp, 549 bp and 420 bp length fragments from sheep, cattle and pig, respectively. This assay had no cross-reaction with isolated DNA from chicken, duck, hare, horse, rat, donkey and fish. This method proved to be sensitive in detecting the presence of low DNA levels at 10 pg in 20 µl PCR mixture. The method was also successfully used for identification of commercial mutton, beef and pork products. It suggested that this method could be used as a reliable routine screening assay for the presence of sheep, cattle and pig ingredients in foodstuffs.

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