Identification by high-performance capillary electrophoresis of wheat lines containing the 1AL.1RS and the 1BL.1RS translocation

Lookhart, G.L.; Bean, S.R.; Graybosch, R.; Chung, O.K.; Morena-Sevilla, B.; Baenziger, S.

Identification by high-performance capillary electrophoresis of wheat lines containing the 1AL.1RS and the 1BL.1RS translocation

1996

High-performance capillary electrophoresis (HPCE) was used to analyze grain proteins from U.S. wheat cultivars carrying 1AL.1RS from Amigo or 1BL.1RS from Kavkaz wheat-rye chromosomal translocations. To date, these are the only 1RS translocations known to occur in U.S. cultivars. Chromosome specific patterns were observed among released cultivars and experimental lines. The method was verified by analyzing protein extracts of the heterogeneous cultivars Nekota and Rawhide and their homogeneous, homozygous non-1RS progeny. The purified 1AL.1RS and 1BL.1RS progenies were derived from Nekota and Rawhide, respectively. HPCE provides a rapid and efficient method for detection of flour or grain derived from 1AL.1RS and 1BL.1RS wheats, and can differentiate the two types of translocations.

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