Isotope fractionation (δ13C, δ15N) and microbial community response in degradation of petroleum hydrocarbons by biostimulation in contaminated soil

Liu, Heng; Wu, Manli; Guo, Xiqian; Gao, Huan; Xu, Yinrui

Isotope fractionation (δ13C, δ15N) and microbial community response in degradation of petroleum hydrocarbons by biostimulation in contaminated soil

2022

Liu, Heng | Wu, Manli | Guo, Xiqian | Gao, Huan | Xu, Yinrui

This study investigated the isotope effects of δ¹³C and δ¹⁵N and microbial response during biodegradation of hydrocarbons by biostimulation with nitrate or compost in the petroleum-contaminated soil. Compost and KNO₃ amendments promoted the total petroleum hydrocarbon (TPH) removal accompanied by a significant increase of Actinobacteria and Firmicutes phyla. Soil alpha diversity decreased after 90 days of biostimulation. An inverse significant carbon isotope effect (εc = 16.6 ± 0.8‰) and strong significant nitrogen isotope effect (εN = −24.20 ± 9.54‰) were shown by the KNO₃ supplementation. For compost amendment, significant carbon and nitrogen isotope effect were εc = 38.8 ± 1.1‰ and εN = −79.49 ± 16.41‰, respectively. A clear difference of the carbon and nitrogen stable isotope fractionation was evident by KNO₃ or compost amendment, which indicated that the mechanisms of petroleum degradation by adding compost or KNO₃ may be different.

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