Harvesting of marine microalgae using cationic cellulose nanocrystals

Verfaillie, An; Blockx, Jonas; Praveenkumar, Ramasamy; Thielemans, Wim; Muylaert, Koenraad

Harvesting of marine microalgae using cationic cellulose nanocrystals

2020

Verfaillie, An | Blockx, Jonas | Praveenkumar, Ramasamy | Thielemans, Wim | Muylaert, Koenraad

Flocculation of marine microalgae is challenging because of the high ionic strength of the culture medium. We investigated cationic cellulose nanocrystals (CNCs) as biobased flocculants for the marine microalgae Nannochloropsis oculata, and compared its performance to chitosan. Cationic CNCs induced flocculation at a low dose of 11 mg L⁻¹, while chitosan required a dose of 35 mg L⁻¹. Our cationic CNCs possess a permanent positive charge, allowing flocculation over a wide pH-range (4 to 10). The CNC maximum flocculation efficiency was 90 %, while chitosan achieved > 95 %, attributed to small flocs remaining in suspension for CNCs. However, centrifugation for 1 min at 180 g or gravity filtration using a 30 μm nylon filter after CNC flocculation resulted in the removal of these small, stable flocs (∼150 μm diam) and > 95% harvesting efficiency. Cationic CNCs can thus serve as a sustainable alternative natural flocculant for harvesting both freshwater and marine microalgae.

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