Specific identification of Pasteurella multocida serogroup-A isolates by PCR assay
2004
Gautam, R. | Kumar, A.A. | Singh, V.P. | Singh, V.P. | Dutta, T.K. | Shivachandra, S.B.
A polymerase chain reaction (PCR) assay targeting the hyaC-hyaD gene was developed and used to identify strains of Pasteurella multocida belonging to serogroup-A. A set of serogroup-specific-PCR primers amplified a 564 bp product from genomic DNA prepared from bacterial cells or directly from bacterial colonies. This method detected as low as 10 ng of bacterial DNA and had a specificity of 100% for P. multocida serogroup-A. A nested PCR method yielded a single 374 bp product. All fifty isolates were also shown to be identical by restriction fragment length polymorphism (RFLP) analysis of the PCR products after digestion with Bg/II.
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