LARGE SCALE BIOSYNTHESIS OF GANGLIOSIDE ANALOGUES BY RERF-LC-AI CELLS CULTURED IN HYPERFlask
2012
Shimura, Yumiko | Suzuki, Junya | Muraoka, Miho | Kasuya, Maria Carmelita Zulueta | Matsuoka, Kōji | Hatanaka, Kenichi
The efficient production of ganglioside analogues was accomplished using RERF-LC-AI cells cultured in HYPERFlask (High Yield PERformance Flask). Eight kinds of ganglioside analogues (GM3, GM2, sialylparagloboside, GD3, di-sialylated lacto-N-tetraose, and another three kinds of analogues with intricate structures) were synthesized by the saccharide primer method using lung squamous-cell carcinoma line RERF-LC-AI and 12-azidododecyl β-lactoside primer. The yield for each analogue obtained using HYPERFlask was higher than yields obtained from 100-mm dishes.
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