Effect of Neuronal PC12 Cells on the Functional Properties of Intestinal Epithelial Caco-2 Cells

SATSU, Hideo; YOKOYAMA, Tatsuya; OGAWA, Nobumasa; FUJIWARA-HATANO, Yoko; Shimizu, Makoto

Effect of Neuronal PC12 Cells on the Functional Properties of Intestinal Epithelial Caco-2 Cells

2003

SATSU, Hideo | YOKOYAMA, Tatsuya | OGAWA, Nobumasa | FUJIWARA-HATANO, Yoko | Shimizu, Makoto

The effect of neuronal cells on the functional properties of intestinal epithelial cells was examined by using an in vitro coculture system. Two cell lines, Caco-2 and PC12, were respectively used as intestinal epithelial and enteric neuronal cell models. Coculture of differentiated Caco-2 cells with PC12 caused a significant decrease in the transepithelial electrical resistance (TER) value of the Caco-2 monolayer. The permeability to lucifer yellow (LY) was also significantly increased, suggesting that the tight junction (TJ) of the Caco-2 monolayers was modulated by coculturing with PC12. To identify the TJ-modulating factor presumably secreted from PC12, the effects of the major neurotransmitters on the TER value and LY transport were examined, but no influence was apparent. The TJ-modulating effect of PC12 was prevented by exposing PC12 to cycloheximide, suggesting that new protein synthesis in PC12 was necessary for this regulation.

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