Chromatographic Separations of Inositol Phosphorus Compounds

Smith, Donald H.; Clark, Francis E.

Chromatographic Separations of Inositol Phosphorus Compounds

1952

Smith, Donald H. | Clark, Francis E.

A chromatographic technique for the separation of sodium phytate and its enzymatically produced derivatives is described. From a mixture of sodium phytate and derivatives, nine organic phosphorus compounds were separated in sufficient quantities to permit determination of their inositol/phosphorus ratios. Phosphorus was determined colorimetrically following perchloric acid digestion, and inositol was determined by means of a biological assay. The determinations were replicated sufficiently that the number of phosphorus atoms per molecule of inositol was considered to have been determined to within one atom of the true value in all cases. The identity of the nine separated compounds was tentatively established.

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