Enzyme activity modification in adult beetles (Agelastica coerulea) inhabiting birch trees in an ozone-enriched atmosphere

Abu ElEla, Shahenda A.; Agathokleous, Evgenios; Ghazawy, Nirvina A.; Amin, Tarek R.; ElSayed, Wael M.; Koike, Takayoshi

Enzyme activity modification in adult beetles (Agelastica coerulea) inhabiting birch trees in an ozone-enriched atmosphere

2018

Tropospheric ozone (O₃) is a naturally occurring gas in the atmosphere. However, the concentration of O₃ increased in the twentieth century. Although the effects of O₃ on vegetation have been extensively studied since the 1950s, limited information exists regarding the effects of O₃ on insect herbivores. In particular, evidence is lacking regarding the effects of O₃ on the biology of insect herbivores. Agelastica coerulea Baly (1874) is a coleopteran species that grazes on Betulaceae plants. In this study, to investigate the effects of O₃ on A. coerulea biology for the first time, female adult insects were collected from Japanese white birch trees grown in a Free Air Controlled Exposure System (FACE) in Sapporo, Japan. These beetles inhabited trees exposed either to ambient or to elevated O₃ for 23 days. After collection, the enzyme activities in the beetles were measured. Elevated O₃ led to a greater total antioxidant activity and lower α- and β-esterase activities, a phenomenon that may suggest an increased resistance of the beetles to stress. Our results are further discussed with regard to biological and toxicological aspects. Collectively, our findings indicate that total antioxidants and α- and β-esterase activities can serve as effective O₃ biomarker systems in this beetle species. This adaptive response of the beetle, which was induced by moderate O₃ exposure, should be further tested across generations and for its protection against greater exposure.

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