Enhancement of the denitrification in low C/N condition and its mechanism by a novel isolated Comamonas sp. YSF15
2020
Su, Jun feng | Yang, Shu | Huang, Ting lin | Li, Min | Liu, Jia ran | Yao, Yi xin
A novel denitrifying bacterium YSF15 was isolated from the Lijiahe Reservoir in Xi’an and identified as Comamonas sp. It exhibited excellent nitrogen removal ability under low C/N conditions (C/N = 2.5) and 94.01% of nitrate was removed in 18 h, with no accumulation of nitrite. PCR amplification and nitrogen balance experiments were carried out, showing that 68.92% of initial nitrogen was removed as gas products and the nitrogen removal path was determined to be NO3−-N→NO2−-N→NO→N2O→N2. Scanning electron microscopy and three-dimensional fluorescence spectroscopy were used to track extracellular polymeric substances (EPS). The results show that complete-denitrification under low C/N conditions is associated with EPS, which may provide a reserve carbon source in extreme environments. These findings reveal that Comamonas sp. YSF15 can provide novel basic materials and a theoretical basis for wastewater bioremediation under low C/N conditions.
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