Acute toxic effects of thiamethoxam on Chinese mitten crab Eriocheir sinensis

Sun, Yue; Yuan, Chunying; Cui, Qingman

Acute toxic effects of thiamethoxam on Chinese mitten crab Eriocheir sinensis

2022

Sun, Yue | Yuan, Chunying | Cui, Qingman

The information about toxic effects of thiamethoxam on non-target aquatic organisms is still incomplete. The semi-static toxicity test method was used to investigate the acute toxic effects of thiamethoxam on Eriocheir sinensis. The results showed that the median lethal concentration (LC50) of thiamethoxam to E. sinensis at 96 h was 510 μg/L, and the safety concentration (SC) was 51 μg/L. After 96 h exposure to thiamethoxam, the survival rates of crabs at concentrations of 0, 151.11, 226.67, 340, and 510 μg/L were 100%, 76.19%, 64.29%, 61.91%, and 46.43%, respectively. A significant (P < 0.05) decrease of the number of hemocytes was observed in thiamethoxam groups. With the increase of thiamethoxam concentration, the phagocytic activity of hemocytes, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and the activities of protease, amylase, and lipase of crabs increased firstly and then decreased, and the above indexes reached the maximum in 151.11 μg/L thiamethoxam group. Collectively, a high concentration of thiamethoxam (510 μg/L) had a great effect on the gene expression of immune metabolism-related factors in hepatopancreas and gill of crabs. These findings indicated that thiamethoxam exposure had the ability to impair immune and metabolic systems and resulted in the reduction of survival rate of crabs.

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