Bacterial host interaction of GFPâlabelled Vibrio anguillarum HIâ610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae

Rekecki, A; Gunasekara, R A Y S A; Dierckens, K; Laureau, S; Boon, N; Favoreel, H; Cornelissen, M; Sorgeloos, P; Ducatelle, R; Bossier, P; Van den Broeck, W

Bacterial host interaction of GFPâlabelled Vibrio anguillarum HIâ610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae

2012

The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V.Â anguillarum with sea bass larvae. In the present study, germâfree sea bass larvae were orally exposed to a V.Â anguillarum HIâ610 pathogen labelled with the green fluorescent protein (GFPâHIâ610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2âh postâexposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48âh p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of midâ and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence.

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