Effects of Phytophthora palmivora on zygotic embryos of papaya in vitro

Sharma, N.K.; Bedi, S.

Effects of Phytophthora palmivora on zygotic embryos of papaya in vitro

1990

Sharma, N.K. | Bedi, S.

Zygotic embryos of Carica papaya were successfully germinated in vitro on Murashige and Skoog's medium supplemented with 2% activated charcoal. The effects of light, temperature, sucrose and nutrient concentrations in the medium, on growth and development of embryos were examined. Strength of the nutrients in the medium had no effect on the growth and development of embryos. The germinated embryos of different varieties of papaya were inoculated using a sporangial suspension of different isolates of Phytophthora palmivora. In the analysis of variance, varieties, isolates and variety—isolate interactions differed significantly. The results were compared with the inoculation of glasshouse-grown seedlings; it is suggested that embryo inoculation could be a useful method of detecting resistance at an early stage of plant development.

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