Intra- and interspecific relationships of veterinary campylobacters revealed by numerical analysis of electrophoretic protein profiles and DNA: DNA hybridizations

Vandamme, P.; Pot, B.; Falsen, E.; Kersters, K.; Ley, J. de

Intra- and interspecific relationships of veterinary campylobacters revealed by numerical analysis of electrophoretic protein profiles and DNA: DNA hybridizations

1990

Vandamme, P. | Pot, B. | Falsen, E. | Kersters, K. | Ley, J. de

The intra- and interspecific relationships of Campylobacter mucosalis and Campylobacter hyointestinalis were studied by numerical analysis of gel electrophoretic protein profiles, DNA: DNA hybridizations and determinations of the DNA base compositions. C. hyointestinalis strains, isolated from porcine, bovine and human sources, showed high DNA binding values (> 64%) and had similar DNA base ratios (33.6 to 35.2 mol%). C. mucosalis strains, representing the different serotypes within this species, also showed high DNA binding values (> 73%) and had similar percentage G + C values (36.0 to 37.4 mol%). Several protein electrophoretic types within both species could be distinguished visually as well as by numerical analysis of the protein profiles. Our results indicate that polyacrylamide gel electrophoresis of whole-cell proteins is a reliable method for typing these strains and might be useful in epidemiological studies.

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